GUARDA IL VIDEO

Quantibody® array is an array-based multiplex sandwich ELISA system for simultaneous quantitative measurement the concentration of multiple cytokines in serum, plasma, CSF, cell lysate, conditioned media and other body fluid. It combines the advantages of the high detection specificity/ sensitivity of ELISA and the high throughput of the arrays. Like a traditional sandwich-based ELISA, it uses a pair of cytokine-specific antibodies for detection. A capture antibody is first bound to the glass surface. After incubation with the sample, the target cytokine is trapped on the solid surface. A second biotin-labeled detection antibody is then added, which can recognize a different isotope of the target cytokine. The cytokine-antibody-biotin complex can then be visualized through the addition of the streptavidin-labeled Fluorescent dye using a laser scanner. Unlike the traditional ELISA, Quantibody products use array format. By arraying multiple cytokine specific capture antibodies onto a glass support, multiplex detection of cytokines in one experiment is made possible.

In detail, one standard glass slide is spotted with 16 wells of identical cytokine antibody arrays. Each antibody, together with the positive and negative control is arrayed in quadruplicate. The slide comes with a holder that includes a 16-well removable gasket that allows for the processing of up to 16 samples on one slide. Four slide holders can be nested into a tray, which matches a standard microplate and allows for automated robotic high-throughput process of 64 arrays simultaneously.

For cytokine quantification, the array specific cytokine standards, whose concentration has been predetermined, are provided to generate a five-point standard curve of each cytokine. In a real experiment, standard cytokines and samples will be assayed in each array simultaneously through a sandwich ELISA procedure. By comparing signals from unknown samples to the standard curve, the unknown cytokine concentration in the samples will be determined.

  Less sample, more data: Only 50 µl sample is needed for quantification of up to 20 cytokines in quadruplicate. This is 80 times more efficient than traditional ELISA. The advantage of Quantibody array is even precious when sample supply is limited. Less time The processing time for one slide is around 4 hours. Because of the high-throughput capability of the array, hundreds of samples can be processed by one technician in a single day.

ELISA detection sensitivity

ELISA is well-known for its sensitivity and specificity in cytokine quantification. Our Quantibody products use sandwich-ELISA based methology and have been tested to have similar detection sensitivity as traditional ELISA.

Reproducibility

Quantibody products use photobleaching resistant Alexa dyes for detection, which greatly increases the system reproducibility and dynamic detection sensitivity. The slides can be rescanned for multiple purposes and stored in dark for months without loss of sensitivity.

Flexibility

Our available kits include either 10 or 20 defined cytokines in the detection panel. However, our custom arrays enable our users to choose not only the cytokine kinds but also the number of cytokines to be included in the array from our several hundred of cytokine pools. In addition, users can choose Alexa 647 dye (red, cy5 channel) for detection instead of Fluorescent dye (green, cy3 channel).

Quantitative analysis software available

Quantibody array-specific Q Analyzer is available for data computation. It is an Excel-based program, which is easy to operate and requires no professional training. Simply copy and paste the raw data into the assigned area. The program will automatically remove the outlier spots and output standard curves and sample cytokine concentrations.